Lysis of whole cells and cell envelopes of Neisseria meningitidis.
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Lysis of whole cells and cell envelopes of Neisseria meningitidis.

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Published .
Written in English


  • Neisseria.

Book details:

The Physical Object
Paginationx, 63 l.
Number of Pages63
ID Numbers
Open LibraryOL16721648M

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Neisseria meningitidis infection alters the polar architecture of the epithelial barrier without disrupting its integrity. In order to evaluate the ability of N. meningitidis to cross an intact epithelium, Calu‐3 cells grown for 10 days on collagen‐coated Transwell inserts were infected at their apical side with the reference MC58 by:   Large-scale screening of Neisseria meningitidis strains is necessary for epidemiological studies as well as for identifying immunologically important antigens. We have developed a new and simpler type of ELISA for this purpose. Whole bacteria from the strains being studied are coated onto PVC plates; the type and subtype are then determined by the binding of monoclonal antibodies with Cited by: Challenge of meningioma cells with live Neisseria meningitidis, isolated outer membranes or pure LOS Meningioma cell monolayers (* 4 cells/well in collagen-coated well tissue culture plates.   LPS, a second major constituent of the cell-envelope of N. meningitidis, is often referred to as endotoxin and plays an important role in virulence. It is held responsible for the severe pathological effects that occur during invasive meningococcal disease. LPS consists of three parts: a lipid A part containing unique hydroxy fatty-acid chains, a core oligosaccharide containing 3-deoxy-D .

Preparation of whole-cell extracts. N. meningitidis strains MC58, MC58 ΔGNA33, BZ, and BZ ΔGNA33 were grown overnight on a GC plate at 37°C in 5% CO 2. Colonies from each strain were collected and used to inoculate 7 ml of Mueller-Hinton broth, containing % glucose, to reach an optical density at nm (OD ) of to Neisseria meningitidis is fastidious and should be grown overnight on horse blood agar from glycerol stocks for a maximum of 16 h before use to ensure minimal mutation rates or downregulation of virulence factors. This may result from the phase variability of some Neisseria genes.N. meningitidis can be cultured successfully in a low‐carbohydrate medium, such as Mueller–Hinton broth (MHB. Meningitis Neisseria meningitidis, Streptococcus. where the binding targets on the cell envelope are usually. of 10 CFU/ml for whole cells and was also validated by SPR. Again. Preparation of whole-cell suspensions. Whole-cell suspensions should not be used for PCR testing. Grow the N. meningitidis isolate to be tested along with an appropriate reference isolate for QC on a BAP for hours at °C with ~5% CO 2 (or in a candle-jar).

Neisseria meningitidis is a commensal organism of the nasopharynx which can occasionally cross this barrier to infect individuals, causing classical meningitis or potentially fatal septicemia. Meningococcal infections are particularly life-threatening in infants once protection from maternal antibodies diminishes (2, 32).Many aspects of the host response to meningococcal infection remain. Introduction. The primary purpose of this page is to provide illustrations of characteristics of N. meningitidis that may aid in differentiating between this, and other, Neisseria species that produce acid from glucose and maltose.. This page is not intended to be a definitive discussion of N. meningitidis infections but to provide information relating to the accurate identification of N. Neisseria meningitidis Overview: Neisseria meningitidis is a Gram-negative diplococcal bacterium responsible for causing meningococcal meningitis (Figure 1). This bacterium is not part of the normal flora, but is found to live in the throat of 5 to 10% of healthy people. Characterization of spheroplast membranes of Neisseria meningitidis group B. Hill JC, Peterson NR, Weiss E. Spheroplast membranes (spheroplast envelopes) of strain of group B Neisseria meningitidis were prepared by a procedure that included lysozyme treatment of the cells and osmotic lysis of the resulting spheroplasts.